Reconstructed Skin Micronucleus Assay (RSMN)
Using the EpiDerm™ Reconstructed Human Epidermis Model
Evaluate the genotoxic potential of new ingredients and chemicals at exposure-relevant doses
RSMN determines the skin genotoxicity/mutagenicity potential of a test substance by determining the relative increase in the induction of micronuclei in test substance- treated tissues compared to negative control-treated tissues
Reconstructed human epidermal tissues may more accurately model the complexity of skin absorption, human epidermal metabolism, and tissue-specific responses than traditional monolayer culture systems
Complex test formulations may be applied in a biologically-relevant fashion directly onto the stratum corneum
Protocols allow for 48 and 72 hour exposures on the metabolically active tissues with no exogenous source of metabolic activation (S9)
RSMN is ideal as a 2nd tier genotoxicity test, and may be used to address positive in vitro mammalian genotoxicity test results
The Institute for In Vitro Sciences, Inc. is unique in its position as a non-profit educational and laboratory resource which possesses many years of practical experience in the application of in vitro testing methods. Members of its technical team have experience in in vitro assay validation studies conducted worldwide, as well as extensive experience in serving the testing needs of diverse industrial clients. Our familiarity with an array of different assay systems and product lines gives us the knowledge and expertise to work with our clients to determine the optimum assay for specific test materials and applications.
Assays are conducted under GLP conditions and according to FDA, EPA, OECD, and other guidelines. All lab personnel go through a rigorous training process in our assay techniques as well as in working in a GLP Environment. Additionally, IIVS follows internal SOPs and established protocols for every study performed.
In vitro assays are performed by the IIVS laboratory in the following research areas: Ocular and Dermal Irritation, Phototoxicity, Dermal Corrosivity, Biological Activity Studies, Acute Systemic Toxicity, and Specialized Investigations. Specialized and/or custom made protocols may be prepared to fit the need of your study. Please contact us for further information.
Select from the menu to the left for more information regarding the individual assays available.